Agglutination test for S. aureus

Agglutination test for S. aureus

Meaning and usage:
Kit - Coagulase test S. AUREUS is intended for quick, easy and accurate identification of Staphylococcus aureus. 
Colonies from blood agar are the starting material for determination.


Principle of the test:
Pathogenic strains of S. aureus produce an enzyme bound coagulase, the so-called clumping factor and the cells of these strains 
contain protein A. Rabbit immunoglobulin (IgG) and fibrinogen are bound to the latex particles of the S. AUREUS LATEX Coagulase Test. 
When the clumping factor reacts with fibrinogen or protein A with IgG, a clear agglutination of the latex suspension occurs. Clumping factor and 
protein A are mutually independent factors and their detection is important in the identification of S. aureus.

Test execution:

1. Put one drop of ANTI S.AUREUS latex suspension on the black field of the detection card.
2. Scoop up about 5 colonies of the tested strain with the provided stirrer and mix them well in the latex suspension.
3. Rock the detection card for one minute. When testing multiple logs at once, be careful not to mix material from adjacent areas. 
If this happens, repeat the test.

Notice:
1. The S. AUREUS coagulation test kit is supplied in a package of 50 tests and is intended for in vitro use only.
2. Store the kit in the dark at 2-8°C and use by the expiry date stated on the package. The latex suspension must not freeze!
3. If the IVD primary packaging is damaged, do not use and contact the manufacturer.
4. Dispose of unused IVD and empty packaging in accordance with the Waste Act no. 185/2001 Coll.